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2018 Vol.43, Issue 4 Preview Page
December 2018. pp. 401-409

Decay of fruit is one of the greatest issues in fruit storage. Purpose: In this study, citrus sterilization was performed to evaluate a dry sterilization method using an atmospheric-pressure nonthermal plasma treatment based on a dielectric-barrier discharge technique. Methods: Citrus samples were stored under four different environmental conditions as follows: group A had cold storage with plasma treatment with a temperature of 6.2 ± 1.0℃ and relative humidity (RH) of 93.4 ± 8.2%, group B had ambient-temperature storage with 22.9 ± 2.3℃ and 82.1 ± 4.5% RH, group C ambient-temperature storage with plasma treatment with 25.3 ± 2.2℃ and 90.0 ± 2.8% RH, and group D had cold storage with 5.7 ± 1.0℃ and 93.4 ± 6.5% RH. Results: As a result of citrus surface sterilization by plasma treatment, treatment groups A and C together showed an average of 16.1 CFU/mL of mold colonies, while control groups B and D showed an average of 2.2ⅹ102 CFU/mL or approximately 13 times greater than the treatment groups. Regarding the mean concentration of aerobic bacteria colonies, the treatment groups (A and C) and control groups (B and D) showed an average of 7.1 CFU/mL and 1.9ⅹ103 CFU/mL, respectively. This is approximately a 270-fold difference in the concentration of pathogen colonies between treatment and control groups. Conclusions: The results showed the potential of nonthermal plasma treatment for citrus storage in enhancing storage duration and quality preservation.

  1. Ahn, K. A., T. W. Moon and B. S. Kim. 2018. An economic review on the short-run storability of field citrus in Jeju. Journal of the Korea Academia-Industrial cooperation Society 19(8): 392-402 (In Korean, with English abstract).
  2. Burdurlu, H. S., N. Koca and F. Karadeniz. 2006. Degradation of vitamin C in citrus juice concentrates during storage. Journal of Food Engineering 74(2): 211-216.10.1016/j.jfoodeng.2005.03.026
  3. Brandenburg, R. 2017. Dielectric barrier discharges: progress on plasma sources and on the understanding of regimes and single filaments. Plasma Sources Science and Technology 27(7): 079501.10.1088/1361-6595/aaced9
  4. Brun, P., P. Brun, M. Vono, P. Venier, E. Tarricone, V. Deligianni, E. Martines, M. Zuin, S. Spagnolo, R. Cavazzana, R. Cardin, I. Castagliuolo, A. L. G. Valerio and A. Leonardi. 2012. Disinfection of ocular cells and tissues by atmospheric-pressure cold plasma. PLoS ONE 7(3):e33245. 10.1371/journal.pone.0033245
  5. Geissmann, Q. 2013. OpenCFU, a new free and open- source software to count cell colonies and other circular objects. PLoS One. 8(2): e54072.10.1371/journal.pone.0054072
  6. Gong, Y. J., Y. Sui, C. S. Han and X. F. Ning. 2016. Drying ginseng slices using a combination of microwave and far-infrared drying techniques. Journal of Biosystems Engineering 41(1): 34-42.10.5307/JBE.2016.41.1.034
  7. Holmes, G. J. and J. W. Eckert. 1999. Sensitivity of Penicillium digitatum and P. italicum to postharvest citrus fungicides in California. Phytopathology 89(9): 716-721.10.1094/PHYTO.1999.89.9.716
  8. Hyun, J. W., S. C. Lee, Y. B. Ihm, D. H. Kim, S. W. Ko and K. S. Kim. 2001. Protective effect of iminoctadinetris (albesilate) and kresoxim-methyl fungicides to citrus postharvest diseases caused by Penicillium spp., The Korean Journal of Pesticide Science 5(2): 37-44 (In Korean, with English abstract).
  9. KREI. 2015. The consumer behavior survey for food. E16-2015. Naju, Republic of Korea: Korea Rural Economic Institute . Available at: (2018.10.1.)(In Korean).
  10. Kim, C. G., H. G. Jeong and D. H. Moon. 2013. Production status and market outlook of domestic and abroad eco-friendly farm products. Final Report of KREI. 2013-55. Naju, Republic of Korea: Korea Rural Economic Institute (In Korean).
  11. Kim, H. G., S. C. Min, D. H. Oh, J. J. Koo and K. B. Song. 2016. Combined treatment of chlorine dioxide gas, mild heat, and fumaric acid on inactivation of Listeria monocytogenes and quality of Citrus unshiu marc. during storage. Journal of the Korean Society of Food Science and Nutrition 45(8): 1233-1238 (In Korean, with English abstract).
  12. Kim, S. -H., J. -H. Lim and J. -S. Koh. 2002. Quality changes of Satsuma during storage by storage warehouse. Korean Journal of Food Preservation 9(2): 131-136 (In Korean, with English abstract).
  13. Ku, K. H., K. -A. Lee and J. H. Choi. 2015. Sensory properties of Satsuma mandarin by quantitative descriptive analysis. Korean Journal of Food Preservation 22(2): 241-250 (In Korean, with English abstract).10.11002/kjfp.2015.22.2.241
  14. Lee, J. -H., H. -J. An, S. -Y. Lee, Y. -H. Choi, B. -S. Lim and Y. -J. Kang. 2009. Changes in quality characteristics of 'Setoka' (Citrus spp.) using different storage methods. Korean Journal of Food Preservation 16(5): 644-649 (In Korean, with English abstract).
  15. MAFRA. 2015. MAFRA Statistics. Sejong, Republic of Korea: Ministry of Agriculture, Food and Rural Affairs. Available at: (2018.10. 2.)(In Korean).
  16. Mok, C. and D. M. Song. 2013. Low-pressure discharge plasma inactivation of Salmonella typhymurium and sanitation of egg. Food Engineering Progress 17(3): 245-250 (In Korean, with English abstract).
  17. Noriega, E., G. Shama, A. Laca, M. Diaz and M.G. Kong. 2011. Cold atmospheric gas plasma disinfection of chicken meat and chicken skin contaminated with Listeria innocua. Food Microbiology 28(7): 1293-1300.
  18. Oehmigen, K., M. Hähnel, R. Brandenburg, C. H. Wilke, K. -D. Weltmann and Th. von. Woedtke. 2010. The role of acidification for antimicrobial activity of atmospheric pressure plasma in liquids. Plasma Process and Polymers 7(3-4): 250-257.10.1002/ppap.200900077
  19. Ramazzina, I., A. Berardinelli, F. Rizzi, S. Tappi, L. Ragni, G. Sacchetti and P. Rocculi. 2015. Effect of cold plasma treatment on physico-chemical parameters and antioxidant activity of minimally processed kiwifruit. Postharvest Biology and Technology 107: 55-65.10.1016/j.postharvbio.2015.04.008
  20. Roig, M. G., J. F. Bello, Z. S. Rivera and J. F. Kennedy. 1999. Studies on the occurrence of non-enzymatic browning during storage of citrus juice. Food Research International 32(9): 609-619.10.1016/S0963-9969(99)00128-3
  21. Seong, J. Y., M. J. Park, K. H. Kwon and S. W. Oh. 2017. Combined effect of cold plasma and UV-C against Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes on fresh-cut lettuce. Journal of Food Hygiene and Safety 32(1): 64-69 (In Korean, with English abstract).
  22. Song, Y. S., Y. R. Park, S. M. Ryu, H. W. Jeon, S. H. Eom and S. J. Lee. 2016. Sterilization and quality variation of dried red pepper by atmospheric pressure dielectric barrier discharge plasma. Korean Journal of Food Preservation 23(7): 960-966 (In Korean, with English abstract).10.11002/kjfp.2016.23.7.960
  23. Yang, Y. T., S. C. Song, S. H. Kim, J. Y. Kim and J. S. Koh. 1997. Cold storage characteristics of early variety of Citrus unshius produced in Cheju with various treatments. Journal of the Korean society of Agricultural Chemistry and Biotechnology 40(2): 117-122 (In Korean, with English abstract).
  24. Won, M. Y., H. Y. Choi, K. S. Lee and S. C. Min. 2016. Helium dielectric barrier discharge-cold plasma treatment for microbiological safety and preservation of onion powder. Korean Society of Food Science and Technology 48(5): 486-491 (In Korean, with English abstract).
  • Publisher :The Korean Society for Agricultural Machinery
  • Publisher(Ko) :한국농업기계학회
  • Journal Title :Journal of Biosystems Engineering
  • Journal Title(Ko) :바이오시스템공학
  • Volume : 43
  • No :4
  • Pages :401-409
  • Received Date :2018. 11. 06
  • Accepted Date : 2018. 12. 03